To provide information about operational parameters at batch configurations such as, the effect of inoculum, addition of nutrients, pH control and substrate concentration to improve hydrogen and ethanol production
According to the results, the selected inoculum was sludge from an aerobic wastewater treatment plant and an aerobic pretreatment. Also, it was necessary to provide nutrients addition and pH control. The substrate concentration that improves hydrogen and ethanol production is in a range of 40 to 70 g glycerol/L. Finally, the results coming from the analysis of the type of inoculum and pretreatment were also used for a continuous system.